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Extraction of Phycocyanin

1. Wall breaking method. Common mechanical wall breaking methods include swelling method, repeated freezing and thawing method, ultrasonic assisted wall breaking method, high pressure homogenization method, tissue grinding method, etc., as well as chemical solvent method, biological enzyme method, etc.Pulsed electric field and resistance heating methods have also been used in cell wall breaking to extract phycocyanin in recent years.

2. Precipitation. After the algae cells are broken, it is very important to choose the appropriate method for precipitation when the phycocyanin is dissolved in the extraction solution. The isoelectric point precipitation method takes advantage of the minimum solubility of the protein at the isoelectric point by adjusting the pH value of the solution to The isoelectric point of phycocyanin causes the solubility of phycocyanin to decrease and cause precipitation.

3. Purification. There are many ingredients in the crude extract of Spirulina, including polysaccharides, proteins, mineral salts, etc., as well as other functional ingredients (chlorophyll, carotene, vitamins, γ-linolenic acid, etc.). The phycocyanin in the crude extract requires Purification to a certain purity can meet different needs. Common phycocyanin purification methods include salting-out precipitation, membrane filtration, aqueous two-phase extraction, free-flow electrophoresis, column chromatography, etc. High-purity phycocyanin can be obtained by the combined use of several purification methods.

1. Antioxidant effect: Phycocyanin has a strong antioxidant effect, which can neutralize free radicals and protect cells from oxidative damage.
2. Lower blood pressure: Phycocyanin has a certain blood pressure lowering effect and can improve the blood pressure level of patients with hypertension.
3. Lower cholesterol: Phycocyanin can lower cholesterol levels and help prevent cardiovascular disease.
4. Improve cognitive function: Phycocyanin can promote the growth and development of nerve cells, help improve cognitive function and prevent Alzheimer’s disease.
5. Improve immunity: Phycocyanin can enhance the activity of immune cells and enhance immunity.
6. Improve exercise performance: Phycocyanin can improve oxygen utilization efficiency, increase exercise endurance and delay fatigue.

Applications of Phycocyanin

Food additives—pigments, as people’s requirements for food safety and health are increasing, phycocyanin has more and more application prospects in the food field. Phycocyanin can be used for coloring and dyeing various foods. Compared with traditional synthetic pigments, phycocyanin is healthier and more natural, and is favored by more and more consumers.Phycocyanincan be used as a natural coloring in foods and beverages, such as blue candies, ice cream, beverages, etc. In October 2021, Youlaiz launched phycocyanin yogurt, which detonated the entire fruit market. Stores rushed to launch new products, and everyone on the street had a cup of blue yogurt. So far, phycocyanin is still one of the most popular brushed yogurts.

Cosmetic field: Phycocyanin can be used as a natural pigment in makeup, skin care products and other cosmetics, giving the product a natural blue color and enhancing the product’s beauty and market competitiveness.

Pharmaceutical field: In a market with growing health demands, phycocyanin is favored by the public for its comprehensive nutrition and rich functions. Phycocyanin has strong antioxidant and anti-inflammatory effects and can be used in the development and production of antioxidants, immune enhancers and other drugs. It can enhance immunity, reduce inflammation, fight cancer, enrich blood, protect liver, protect ovaries, and increase white blood cells. The effect is remarkable and it has high medicinal value.

Biotechnology field: Phycocyanin can be used in the field of biotechnology as a biofluorescent probe, protein stain, etc., and is of great help to life science research.

Environmental protection field: Phycocyanin can be used in water quality testing, environmental pollution monitoring and other fields, and has the characteristics of high sensitivity and high selectivity.

Energy field: Phycocyanin can be used in solar cells and biofuel cells, with high photoelectric conversion efficiency and electrochemical activity.

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Post time: Apr-02-2024